Two Malic Enzymes in Pseudomonas aeruginosa

Oxygen-dependent alginate synthesis and enzymes in Pseudomonas aeruginosa.

Alginate production by the highly alginate-producing Pseudomonas aeruginosa 8821M was maximal at a dissolved oxygen tension (DOT) of 5% of air saturation. Lower DOT limited growth and alginate synthesis. At higher DOT values up to 70% of air saturation, the specific alginate production rate decreased. Nevertheless, the molecular mass of the alginate increased at higher aerations, as indicated b...

Induction of histidine-degrading enzymes in Pseudomonas aeruginosa.

Urocanate but not histidine was able to induce formation of histidine-degrading enzymes in a histidine ammonia-lyase-deficient mutant of Pseudomonas aeruginosa. The results, in conjunction with others reported previously, indicate that urocanate, the first intermediate, is the physiological inducer of the pathway.

Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.

Elastase of Pseudomonas aeruginosa is synthesized as a preproenzyme. The signal sequence is cleaved off during transport across the inner membrane and, in the periplasm, proelastase is further processed. We demonstrate that the propeptide and the mature elastase are both secreted but that the propeptide is degraded extracellularly. In addition, reduction of the extracellular proteolytic activit...

Encapsulated Pseudomonas Aeruginosa (pseudomonas Aeruginosa Mucosus) Strains.

Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes

Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carbapenemase enzymes. Materials and methods: A total of 97 clinical specimens was collected from pa...